Rabbit anti-PSMA1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-845A maps to a region between residue 163 and 213 of human Proteasome (Prosome, Macropain) Subunit, Alpha Type, 1 using the numbering given in entry NP_002777.1 (GeneID 5682).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PSMA1 immobilized on solid support.
The epitope recognized by A303-845A-T maps to a region between residue 163 and 213 of human Proteasome (Prosome, Macropain) Subunit, Alpha Type, 1 using the numbering given in entry NP_002777.1 (GeneID 5682).
Additional Product Information
PSMA1 [proteasome (prosome, macropain) subunit, alpha type, 1] is a subunit of the proteasome, a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA1 is a member of the peptidase T1A family that is a 20S core alpha subunit [taken from NCBI Entrez Gene (Gene ID: 5682)].
Alternate Names
30 kDa prosomal protein; epididymis secretory protein Li 275; HC2; HEL-S-275; macropain subunit C2; macropain subunit nu; multicatalytic endopeptidase complex subunit C2; NU; PROS30; PROS-30; proteasome (prosome, macropain) subunit, alpha type, 1; proteasome component C2; proteasome nu chain; proteasome subunit alpha type-1; proteasome subunit nu; proteasome subunit, alpha-type, 1; protein P30-33K; testicular tissue protein Li 150
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).