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Rabbit anti-PTPN12 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PTPN12
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 400 and 450
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PTPN12 immobilized on solid support.

The epitope recognized by A301-299A maps to a region between residue 375 and 425 of human protein tyrosine phosphatase, non-receptor type 12 using the numbering given in entry NP_002826.2 (GeneID 5782).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PTPN12 immobilized on solid support.

The epitope recognized by A301-299A-T maps to a region between residue 375 and 425 of human protein tyrosine phosphatase, non-receptor type 12 using the numbering given in entry NP_002826.2 (GeneID 5782).
Country of Origin
USA

Additional Product Information

PTPN12 is a cytosolic protein tyrosine phosphatase (PTP) that contains a C-terminal PEST motif and plays an important role in cytoskeletal organization, immune cell activation, apoptosis, and embryonic development. PTPN12 appears to contribute to these cellular processes through its interaction with a variety of signaling and cytoskeletal-associated proteins. The recent discovery that PTPN12 is modulated by caspase-3 cleavage has led to the analysis of the role of PTPN12 and other PTPs as regulators of apoptosis.

5782
PTPN12
protein tyrosine phosphatase, non-receptor type 12
Q05209
Tyrosine-protein phosphatase non-receptor type 12

Alternate Names

protein-tyrosine phosphatase G1; PTPG1; PTP-PEST; tyrosine-protein phosphatase non-receptor type 12

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.