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Rabbit anti-PTRF Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PTRF
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 275 and 325
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PTRF immobilized on solid support.

The epitope recognized by A301-271A maps to a region between residue 238 and 288 human RNA polymerase I and transcript release factor using the numbering given in entry NP_036364.2 (GeneID 284119).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PTRF immobilized on solid support.

The epitope recognized by A301-271A-T maps to a region between residue 238 and 288 human RNA polymerase I and transcript release factor using the numbering given in entry NP_036364.2 (GeneID 284119).
Country of Origin
USA

Additional Product Information

PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis.

284119
CAVIN1
caveolae associated protein 1
Q6NZI2
Polymerase I and transcript release factor

Alternate Names

caveolae-associated protein 1; CAVIN; cavin-1; CGL4; congenital generalized lipodystrophy 4; FKSG13; polymerase I and transcript release factor; PTRF; RNA polymerase I and transcript release factor; TTF-I interacting peptide 12

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.