Rabbit anti-Pur-beta Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-650A-T maps to a region between residue 262 and 312 of human Purine-rich Element Binding Protein B using the numbering given in entry NP_150093.1 (GeneID 5814).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pur-beta immobilized on solid support.
The epitope recognized by A303-650A maps to a region between residue 262 and 312 of human Purine-rich Element Binding Protein B using the numbering given in entry NP_150093.1 (GeneID 5814).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Pur-beta (purine-rich element binding protein B) is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of the Pur-beta gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia [taken from NCBI Entrez Gene (Gene ID: 5814)].
Alternate Names
PURBETA; Purine-rich element-binding protein B; transcriptional activator protein Pur-beta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.