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Rabbit anti-RAP74 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
RAP74
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 350 and 400
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Bovine, Rat, Mouse
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to RAP74 immobilized on solid support.

The epitope recognized by A302-757A-T maps to a region between residue 350 and 400 of human Transcription Initiation Factor RAP74 using the numbering given in entry NP_002087.2 (GeneID 2962).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

The general transcription factor, TFIIF, is composed of a heterodimer of a 74 kDa alpha subunit and a 30 kDa beta subunit. The alpha subunit of TFIIF is also called RAP74. In collaboration with TFIIB, TFIIF helps recruit RNA polymerase II to the pre-initiation complex (PIC).

2962

GTF2F1

general transcription factor IIF subunit 1

P35269

General transcription factor IIF subunit 1

Alternate Names

BTF4, general transcription factor IIF 74 kDa subunit, general transcription factor IIF subunit 1, general transcription factor IIF, polypeptide 1, 74kDa, RAP74, TF2F1, TFIIF, TFIIF-alpha, transcription initiation factor IIF subunit alpha, transcription initiation factor RAP74

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE.