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Rabbit anti-RBM25/RED120 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RBM25/RED120
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 200 and 250
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RBM25/RED120 immobilized on solid support.

The epitope recognized by A301-067A maps to a region between residue 150 and 200 of human RNA binding motif protein 25 (Arg/Glu/Asp-rich protein, 120 kDa) using the numbering given in entry AAF19255.1 (GeneID 58517).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RBM25/RED120 immobilized on solid support.

The epitope recognized by A301-067A-T maps to a region between residue 150 and 200 of human RNA binding motif protein 25 (Arg/Glu/Asp-rich protein, 120 kDa) using the numbering given in entry AAF19255.1 (GeneID 58517).
Country of Origin
USA

Additional Product Information

RBM25, also identified as hRED120 (for human Arg/Glu/Asp-rich protein of 120 kDa), contains an RNA recognition motif and a PWI nucleic acid binding domain and is suggested to have a role in RNA processing.

58517
RBM25
RNA binding motif protein 25
P49756
RNA-binding protein 25

Alternate Names

arg/Glu/Asp-rich protein of 120 kDa; Arg/Glu/Asp-rich protein, 120 kDa; fSAP94; functional spliceosome-associated protein 94; NET52; Protein S164; RED120; RNA-binding motif protein 25; RNA-binding protein 25; RNA-binding region (RNP1, RRM) containing 7; RNA-binding region-containing protein 7; RNPC7; S164; Snu71; U1 small nuclear ribonucleoprotein 1SNRP homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.