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Rabbit anti-RENT1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RENT1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1079 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RENT1 immobilized on solid support.

The epitope recognized by A301-902A maps to a region between residue 1079 and 1129 of human regulator of nonsense transcripts 1 using the numbering given in entry NP_002902.2 (GeneID 5976).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RENT1 immobilized on solid support.

The epitope recognized by A301-902A-T maps to a region between residue 1079 and 1129 of human regulator of nonsense transcripts 1 using the numbering given in entry NP_002902.2 (GeneID 5976).
Country of Origin
USA

Additional Product Information

RENT1, also known as UPF1, is a key regulator of the nonsense-mediated mRNA decay (NMD) pathway. The NMD pathway eliminates mRNAs that prematurely terminate during translation and may serve to protect the cells from dominant-negative effects of truncated proteins, or as a means to regulate protein expression. RENT1 is an ATP-dependent 5’- 3’ RNA helicase and ATPase that localizes to the cytoplasm and is part of a post-splicing multiprotein complex as well as an mRNA decay complex.

5976
UPF1
UPF1, RNA helicase and ATPase
Q92900
Regulator of nonsense transcripts 1

Alternate Names

ATP-dependent helicase RENT1; delta helicase; HUPF1; nonsense mRNA reducing factor 1; NORF1; pNORF1; regulator of nonsense transcripts 1; RENT1; smg-2; smg-2 homolog, nonsense mediated mRNA decay factor; UPF1 regulator of nonsense transcripts homolog; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; yeast Upf1p homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.