Bethyl Laboratories
Rabbit anti-RFWD3 Antibody Affinity Purified
Primary
Catalog #A301-398A
TargetRFWD3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
RFWD3 (ring finger and WD repeat domain 3) has been identified as a protein that is phosphorylated in response to DNA damage. The exact function of RFWD3 (ring finger and WD repeat domain 3) has not been characterized. Structurally, RFWD3 possesses a RING-type zinc finger and 3 WD repeats.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 724 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetRFWD3
Antigen Species
Human
Gene ID55159
Gene Symbol
RFWD3
Gene Name
ring finger and WD repeat domain 3
Uniprot IDQ6PCD5
Protein Name
E3 ubiquitin-protein ligase RFWD3
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RFWD3 immobilized on solid support.
The epitope recognized by A301-398A maps to a region between residue 724 and774 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-398A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-398A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-398AOpen
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