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Rabbit anti-RNF138 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
RNF138
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 1 and 50
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to RNF138 immobilized on solid support.

The epitope recognized by A302-993A-T maps to a region between residue 1 and 50 of human Ring Finger Protein 138 using the numbering given in entry NP_057355.2 (GeneID 51444).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

RING-finger protein 138 (RNF138) is an E3 ubiquitin ligase. Together with NLK, RNF138 is involved in the ubiquitination and degradation of TCF/LEF. RNF138 also exhibits auto-ubiquitination activity in combination with UBE2K. RNF138 may act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8WVD3].

51444

RNF138

ring finger protein 138

Q8WVD3

E3 ubiquitin-protein ligase RNF138

Alternate Names

E3 ubiquitin-protein ligase RNF138, hNARF, HSD-4, NARF, nemo-like kinase associated ring finger protein, Nemo-like kinase-associated RING finger protein, NLK-associated RING finger protein, RING finger protein 138, ring finger protein 138, E3 ubiquitin protein ligase, RING-type E3 ubiquitin transferase RNF138, STRIN, testicular tissue protein Li 165

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE.