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Rabbit anti-RNF31 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RNF31
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1022 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)


$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RNF31 immobilized on solid support.

The epitope recognized by A303-560A maps to a region between residue 1022 and 1072 of human Ring Finger Protein 31 using the numbering given in entry NP_060469.4 (GeneID 55072).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RNF31 immobilized on solid support.

The epitope recognized by A303-560A-T maps to a region between residue 1022 and 1072 of human Ring Finger Protein 31 using the numbering given in entry NP_060469.4 (GeneID 55072).
Country of Origin
USA

Additional Product Information

RING finger protein 31 is an E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96EP0].

55072
RNF31
ring finger protein 31
Q96EP0
E3 ubiquitin-protein ligase RNF31

Alternate Names

E3 ubiquitin-protein ligase RNF31; HOIL-1-interacting protein; HOIP; Paul; RING finger protein 31; RING-type E3 ubiquitin transferase RNF31; ZIBRA; zinc in-between-RING-finger ubiquitin-associated domain protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.