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Primary Antibodies  

Rabbit anti-SAFB1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (5)
Target:

SAFB1

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 775 and 825

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Scaffold attachment factor B1 (SAFB1) is a chromatin associated protein that interacts with DNA elements termed S/MARs (scaffold or matrix attachment regions) but is not part of the nuclear matrix. SAFB1 is ubiquitously expressed and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. SAFB1 has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.

6294

SAFB

scaffold attachment factor B

Q15424

Scaffold attachment factor B1

Alternate Names

glutathione S-transferase fusion protein; HAP; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein; HET; Hsp27 ERE-TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAB-B1; SAF-B; SAFB1; SAF-B1; scaffold attachment factor B1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.