Bethyl Laboratories
Rabbit anti-Sec31A Antibody Affinity Purified
Primary
Catalog #A302-336A
TargetSec31A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 11 hours and 49 minutes for shipment by Tuesday, November 3
Product Description
Sec31A is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function [taken from NCBI GeneID:22872].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 800 and 850
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSec31A
Antigen Species
Human
Gene ID22872
Gene Symbol
SEC31A
Gene Name
SEC31 homolog A, COPII coat complex component
Uniprot IDO94979
Protein Name
Protein transport protein Sec31A
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Sec31A immobilized on solid support.
The epitope recognized by A302-336A maps to a region between residue 775 and 825 of human SEC31 homolog A using the numbering given in entry NP_001070675.1 (GeneID 22872).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-336A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-336A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-336AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1