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Rabbit anti-SENP3 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
SENP3
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 1 and 50
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Antigen Species
Human
Stock Concentration
100 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to SENP3 immobilized on solid support.

The epitope recognized by A303-139A-T maps to a region between residue 1 and 50 of human SUMO1/Sentrin/SMT3 Specific Peptidase 3 using the numbering given in entry NP_056485.2 (GeneID 26168).

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Gene & Protein Information

Sentrin-specific protease 3 (SENP3) functions as a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates.SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability, and also deconjugates SUMO2 and SUMO3 from CDCA8. SENP3 can function as a redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. SENP3 is required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9H4L4].

26168

SENP3

SUMO specific peptidase 3

Q9H4L4

Sentrin-specific protease 3

Alternate Names

sentrin/SUMO-specific protease 3, Sentrin/SUMO-specific protease SENP3, sentrin-specific protease 3, SMT3IP1, SSP3, SUMO1/sentrin/SMT3 specific peptidase 3, SUMO-1-specific protease 3, Ulp1

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.