Rabbit anti-Sirt1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Sirt1 is one of seven members of the sirtuin family of proteins that are homologous to the yeast silent information regulator 2 (Sir2), a NAD+-dependent protein deacetylase that plays a role in lifespan in lower eukaryotes. The sirtuins are diverse in their cellular functions, and are important to the regulation of oxidative stress, and metabolism. Sirt 1 is localized to the nucleus and possesses deacetylase activity. Sirt 1 has been shown to be able to deacetylate p53, Ku70, NF-kappaB and forkhead proteins to mediate cellular resistance to stress.
Alternate Names
hSIR2; hSIRT1; NAD-dependent protein deacetylase sirtuin-1; regulatory protein SIR2 homolog 1; SIR2; SIR2alpha; SIR2L1; SIR2-like protein 1; sirtuin type 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.