Rabbit anti-SMAP Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A304-688A maps to a region between residue 125 to 175 of human Small Acidic Protein using the numbering given in entry NP_055082.1 (GeneID 10944).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SMAP immobilized on solid support.
The epitope recognized by A304-688A-T maps to a region between residue 125 to 175 of human Small Acidic Protein using the numbering given in entry NP_055082.1 (GeneID 10944).
Additional Product Information
Small acidic protein (SMAP) belongs to the SMAP family [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O00193].
Alternate Names
IMAGE145052; small acidic protein; SMAP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.