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Rabbit anti-SMAP1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SMAP1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 275 and 325
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SMAP1 immobilized on solid support.

The epitope recognized by A305-674A maps to a region between residue 275 to 325 of human Stromal membrane-associated protein 1 using the numbering given in entry Q8IYB5.2 (GeneID 60682).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to SMAP1 immobilized on solid support.

The epitope recognized by A305-674A-T maps to a region between residue 275 to 325 of human Stromal membrane-associated protein 1 using the numbering given in entry Q8IYB5.2 (GeneID 60682).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Stromal membrane-associated protein 1 (SMAP1) is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells [taken from NCBI Entrez Gene (Gene ID: 60682)].

60682
SMAP1
small ArfGAP 1
Q8IYB5
Stromal membrane-associated protein 1

Alternate Names

SMAP-1; stromal membrane-associated GTPase-activating protein 1; stromal membrane-associated protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.