Rabbit anti-SMAP1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A305-674A maps to a region between residue 275 to 325 of human Stromal membrane-associated protein 1 using the numbering given in entry Q8IYB5.2 (GeneID 60682).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SMAP1 immobilized on solid support.
The epitope recognized by A305-674A-T maps to a region between residue 275 to 325 of human Stromal membrane-associated protein 1 using the numbering given in entry Q8IYB5.2 (GeneID 60682).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Stromal membrane-associated protein 1 (SMAP1) is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells [taken from NCBI Entrez Gene (Gene ID: 60682)].
Alternate Names
SMAP-1; stromal membrane-associated GTPase-activating protein 1; stromal membrane-associated protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.