Rabbit anti-SMARCC1/BAF155 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A301-019A maps to a region between residue 300 and 350 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1/BRG1-associated factor 155 using the numbering given in entry NP_003065.2 (GeneID 6599).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SMARCC1/BAF155 immobilized on solid support.
The epitope recognized by A301-019A-T maps to a region between residue 300 and 350 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1/BRG1-associated factor 155 using the numbering given in entry NP_003065.2 (GeneID 6599).
Additional Product Information
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCC1/BAF155 is part of the BAF complex that associates with SMARCA2/Brm and SMARCA4/Brg. The mouse homolog is Srg3 and has been found to be required for embryogenesis and brain development.
Alternate Names
BAF155; BRG1-associated factor 155; chromatin remodeling complex BAF155 subunit; CRACC1; mammalian chromatin remodeling complex BRG1-associated factor 155; Rsc8; SRG3; SWI/SNF complex 155 kDa subunit; SWI/SNF complex subunit SMARCC1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SWI3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.