Rabbit anti-SMC2 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core condensin complex includes a heterodimer of SMC2 and SMC4 that functions to create positively supercoiled loops for chromatin condensation and metaphase chromatid shaping. A function for condensin in the DNA damage checkpoint response and gene repression has also been suggested.
Alternate Names
CAPE; CAP-E; chromosome-associated protein E; hCAP-E; SMC protein 2; SMC-2; SMC2 (structural maintenance of chromosomes 2, yeast)-like 1; SMC2L1; structural maintenance of chromosomes (SMC) family member, chromosome-associated protein E; structural maintenance of chromosomes protein 2; XCAP-E homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.