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Rabbit anti-SPCS2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SPCS2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Dog
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SPCS2 immobilized on solid support.

The epitope recognized by A305-608A maps to a region between residue 25 to 75 of human Signal peptidase complex subunit 2 using the numbering given in entry Q15005.3 (GeneID 9789).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to SPCS2 immobilized on solid support.

The epitope recognized by A305-608A-T maps to a region between residue 25 to 75 of human Signal peptidase complex subunit 2 using the numbering given in entry Q15005.3 (GeneID 9789).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Signal peptidase complex subunit 2 (SPCS2) is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15005].

9789
SPCS2
signal peptidase complex subunit 2
Q15005
Signal peptidase complex subunit 2

Alternate Names

microsomal signal peptidase 25 kDa subunit; signal peptidase 25kDa subunit; signal peptidase complex subunit 2; signal peptidase complex subunit 2 homolog; SPase 25 kDa subunit

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.