Rabbit anti-SPCS2 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A305-608A maps to a region between residue 25 to 75 of human Signal peptidase complex subunit 2 using the numbering given in entry Q15005.3 (GeneID 9789).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SPCS2 immobilized on solid support.
The epitope recognized by A305-608A-T maps to a region between residue 25 to 75 of human Signal peptidase complex subunit 2 using the numbering given in entry Q15005.3 (GeneID 9789).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Signal peptidase complex subunit 2 (SPCS2) is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15005].
Alternate Names
microsomal signal peptidase 25 kDa subunit; signal peptidase 25kDa subunit; signal peptidase complex subunit 2; signal peptidase complex subunit 2 homolog; SPase 25 kDa subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.