Rabbit anti-SPG20 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-375A maps to a region between residue 350 to 400 of human Spartin using the numbering given in entry Q8N0X7.1 (GeneID 23111).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SPG20 immobilized on solid support.
The epitope recognized by A305-375A-T maps to a region between residue 350 to 400 of human Spartin using the numbering given in entry Q8N0X7.1 (GeneID 23111).
Additional Product Information
Spastic paraplegia 20 protein (SPG20) is a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Mutations associated with the SPG20 gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome) [taken from NCBI Entrez Gene (Gene ID: 23111)].
Alternate Names
spartin; spastic paraplegia 20 (Troyer syndrome); Spastic paraplegia 20 protein; SPG20; TAHCCP1; trans-activated by hepatitis C virus core protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.