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Primary Antibodies  

Rabbit anti-SUPT5H Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (6)
Target:

SUPT5H

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 50 and 100

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Transcription elongation is one step in the multistage process of transcription that involves the polymerization of nucleoside monophosphates into a ribonucleotide transcript catalyzed by the multisubunit complex RNA Pol II. Transcriptional regulation involves positive factors that promote sufficient elongation rates and processivity as well as negative elongation factors that repress transcription elongation. SUPT5H is a subunit of the DRB sensitivity inducing factor (DSIF) along with SUPT4H that functions as a negative elongation factor. DSIF associates with RNA Pol II and may inhibit elongation by pausing and terminating RNA Pol II activity.

6829

SUPT5H

SPT5 homolog, DSIF elongation factor subunit

O00267

Transcription elongation factor SPT5

Alternate Names

DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; hSPT5; SPT5; SPT5H; suppressor of Ty 5 homolog; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; transcription elongation factor SPT5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.