Rabbit anti-TGFBR2 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A304-413A-T maps to a region between residue 475 to 525 of human Transforming Growth Factor, Beta Receptor II using the numbering given in entry NP_003233.4 (GeneID 7048).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TGFBR2 immobilized on solid support.
The epitope recognized by A304-413A maps to a region between residue 475 to 525 of human Transforming Growth Factor, Beta Receptor II using the numbering given in entry NP_003233.4 (GeneID 7048).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TGF-beta type II receptor (TGFBR2) is a transmembrane serine/threonine kinase that complexes with the TGF-beta type I serine/threonine kinase receptor (TGFBR1) to form the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. The TGFBR complex transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade.The TGFBR is also involved in non-canonical, SMAD-independent TGF-beta signaling pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P37173].
Alternate Names
AAT3; FAA3; LDS1B; LDS2; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TBRII; TBR-ii; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta type II receptor; TGFbeta-RII; TGFR-2; transforming growth factor beta receptor II; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II alpha; transforming growth factor, beta receptor II beta; transforming growth factor, beta receptor II delta; transforming growth factor, beta receptor II epsilon; transforming growth factor, beta receptor II gamma; Transforming growth factor-beta receptor type II
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.