Rabbit anti-TIF-IA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-065A maps to a region between residue 601 and 651 of human Transcription Initiation Factor IA using the numbering given in entry NP_060897.3 (GeneID 54700).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Transcription initiation factor IA (TIF-IA) is encoded by the RRN3 gene which was identified and cloned due to its homology with the yeast Rrn3p gene. TIF-IA has been shown to recruit initiation-competent RNA Polymerase I to rRNA promoters.
Alternate Names
A-270G1.2; RNA polymerase I-specific transcription initiation factor RRN3; RRN3 RNA polymerase I transcription factor homolog; TIFIA; TIF-IA; transcription initiation factor IA; transcription initiation factor TIF-IA
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.