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Rabbit anti-TNIK Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
TNIK
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 600 and 650
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to TNIK immobilized on solid support.

The epitope recognized by A302-695A maps to a region between residue 600 and 650 of human TRAF2 and NCK interacting kinase using the numbering given in entry NP_055843.1 (GeneID 23043).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to TNIK immobilized on solid support.

The epitope recognized by A302-695A-T maps to a region between residue 600 and 650 of human TRAF2 and NCK interacting kinase using the numbering given in entry NP_055843.1 (GeneID 23043).
Country of Origin
USA

Additional Product Information

TNIK (TRAF2 and NCK-interacting protein kinase) is a serine threonine kinase that acts as an essential activator of the Wnt signaling pathway. TNIK is recruited to promoters of Wnt target genes and is required to activate Wnt target genes expression. TNIK may also play a role in the response to environmental stress and appears to act upstream of the JUN N-terminal pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UKE5].

23043
TNIK
TRAF2 and NCK interacting kinase
Q9UKE5
TRAF2 and NCK-interacting protein kinase

Alternate Names

MRT54; TRAF2 and NCK-interacting protein kinase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.