Rabbit anti-TOM1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-361A-T maps to a region between residue 400 and 450 of human target of myb1 using the numbering given in entry NP_005479.1 (GeneID 10043).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TOM1 immobilized on solid support.
The epitope recognized by A301-361A maps to a region between residue 400 and 450 of human target of myb1 using the numbering given in entry NP_005479.1 (GeneID 10043).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TOM1 (target of myb1) was originally identified as a gene activated by the expression of oncogenic Myb1. TOM1 has been shown to form a complex with tollip (toll-interacting protein) and to bind ubiquitin chains. It has also been shown to bind to clathrin heavy chain and is proposed to function to link polyubiquitinated proteins to clathrin and regulate endosomal trafficking of ubiquitinated proteins. Recent evidence shows that TOM1 bridges endofin to clathrin and is critical for the endosomal targeting of clathrin by endofin.
Alternate Names
target of myb 1; target of Myb protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.