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Rabbit anti-TTK Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
TTK
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 807 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to TTK immobilized on solid support.

The epitope recognized by A300-296A-T maps to a region between residue 800 and c-terminus (residue 841) of human TTK using the numbering given Swiss-Prot entry P33981 (GeneID 7272).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to TTK immobilized on solid support.

The epitope recognized by A300-296A maps to a region between residue 800 and c-terminus (residue 841) of human TTK using the numbering given Swiss-Prot entry P33981 (GeneID 7272).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

TTK is a cell-cycle regulated dual-specificity kinase that is part of the Mps1 family of kinases. TTK has been shown to interact with Chk2, a cell cycle checkpoint kinase, to mediate cellular responses to DNA damage. TTK has also been shown to be localized from the kinetochore to the centrosome at the entrance of anaphase, and required for mitotic spindle maintenance through the interaction with TACC2.

7272
TTK
TTK protein kinase
P33981
Dual specificity protein kinase TTK

Alternate Names

cancer/testis antigen 96; CT96; dual specificity protein kinase TTK; ESK; monopolar spindle 1 kinase; monopolar spindle 1-like 1; MPH1; MPS1; MPS1L1; phosphotyrosine picked threonine kinase; phosphotyrosine picked threonine-protein kinase; PYT

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.