Rabbit anti-TTK Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A300-296A-T maps to a region between residue 800 and c-terminus (residue 841) of human TTK using the numbering given Swiss-Prot entry P33981 (GeneID 7272).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TTK immobilized on solid support.
The epitope recognized by A300-296A maps to a region between residue 800 and c-terminus (residue 841) of human TTK using the numbering given Swiss-Prot entry P33981 (GeneID 7272).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TTK is a cell-cycle regulated dual-specificity kinase that is part of the Mps1 family of kinases. TTK has been shown to interact with Chk2, a cell cycle checkpoint kinase, to mediate cellular responses to DNA damage. TTK has also been shown to be localized from the kinetochore to the centrosome at the entrance of anaphase, and required for mitotic spindle maintenance through the interaction with TACC2.
Alternate Names
cancer/testis antigen 96; CT96; dual specificity protein kinase TTK; ESK; monopolar spindle 1 kinase; monopolar spindle 1-like 1; MPH1; MPS1; MPS1L1; phosphotyrosine picked threonine kinase; phosphotyrosine picked threonine-protein kinase; PYT
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.