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Rabbit anti-UBAP1/NAG20 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
UBAP1/NAG20
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 25 and 75
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to UBAP1/NAG20 immobilized on solid support.

The epitope recognized by A304-848A-T maps to a region between residue 25 to 75 of human Ubiquitin-associated protein 1 using the numbering given in entry Q9NZ09.1 (GeneID 51271).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to UBAP1/NAG20 immobilized on solid support.

The epitope recognized by A304-848A maps to a region between residue 25 to 75 of human Ubiquitin-associated protein 1 using the numbering given in entry Q9NZ09.1 (GeneID 51271).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Ubiquitin-associated protein 1 (UBAP1) is a component of the ESCRT-I complex, a regulator of vesicular trafficking process. It binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). It plays a role in the proteasomal degradation of ubiquitinated cell-surface proteins, such as EGFR and BST2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NZ09].

51271
UBAP1
ubiquitin associated protein 1
Q9NZ09
Ubiquitin-associated protein 1

Alternate Names

NAG20; nasopharyngeal carcinoma-associated gene 20 protein; UAP; UBAP; UBAP-1; ubiquitin-associated protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.