Rabbit anti-UBE2T Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-874A maps to a region between residue 147 and 197 of human ubiquitin-conjugating enzyme E2T using the numbering given in entry NP_054895.1 (GeneID 29089).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBE2T immobilized on solid support.
The epitope recognized by A301-874A-T maps to a region between residue 147 and 197 of human ubiquitin-conjugating enzyme E2T using the numbering given in entry NP_054895.1 (GeneID 29089).
Additional Product Information
Ubiquitin-conjugating enzyme E2 T (UBE2T) is an E2 ubiquitin-conjugating enzyme that is part of the three-step pathway responsible for the covalent attachment of ubiquitin to proteins for their targeted degradation. UBET2 in conjuction with the Fanconi anemia (FA) complex is responsible for the DNA damage-induced monoubiquitination of the FANCD2 protein of the Fanconi Anemia pathway.
Alternate Names
cell proliferation-inducing gene 50 protein; E2 ubiquitin-conjugating enzyme T; FANCT; HSPC150; HSPC150 protein similar to ubiquitin-conjugating enzyme; PIG50; ubiquitin carrier protein T; ubiquitin conjugating enzyme E2T; ubiquitin-conjugating enzyme E2 T; ubiquitin-conjugating enzyme E2T (putative); ubiquitin-protein ligase T
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.