Rabbit anti-UBR7 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A304-130A maps to a region between residue 1 to 50 of human Ubiquitin Protein Ligase E3 component N-Recognin 7 using the numbering given in entry NP_786924.2 (GeneID 55148).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBR7 immobilized on solid support.
The epitope recognized by A304-130A-T maps to a region between residue 1 to 50 of human Ubiquitin Protein Ligase E3 component N-Recognin 7 using the numbering given in entry NP_786924.2 (GeneID 55148).
Additional Product Information
Putative E3 ubiquitin-protein ligase UBR7 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. UBR7 recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N806].
Alternate Names
C14orf130; N-recognin-7; putative E3 ubiquitin-protein ligase UBR7; RING-type E3 ubiquitin transferase UBR7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.