Rabbit anti-UTX Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-374A maps to a region between residue 700 and 750 of human ubiquitously transcribed tetratricopeptide repeat, X chromosome using the numbering given in entry NP_066963.1 (GeneID 7403).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UTX immobilized on solid support.
The epitope recognized by A302-374A-T maps to a region between residue 700 and 750 of human ubiquitously transcribed tetratricopeptide repeat, X chromosome using the numbering given in entry NP_066963.1 (GeneID 7403).
Additional Product Information
UTX (ubiquitously transcribed X chromosome tetratricopeptide repeat protein) is a JmjC domain- and tetratricopeptide repeat (TPR) domain- containing protein. UTX functions as a histone demethylase that specifically demethylates 'Lys-27' of histone H3, It demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. UTX plays a central role in regulation of posterior development, by regulating HOX gene expression [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O15550].
Alternate Names
bA386N14.2; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)); histone demethylase UTX; KABUK2; lysine (K)-specific demethylase 6A; lysine-specific demethylase 6A; ubiquitously transcribed tetratricopeptide repeat protein X-linked; ubiquitously-transcribed TPR gene on the X chromosome; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein; UTX
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.