Rabbit anti-VBP1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A305-403A-T maps to a region between residue 147 to 197 of human Prefoldin subunit 3 using the numbering given in entry P61758.3 (GeneID 7411).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to VBP1 immobilized on solid support.
The epitope recognized by A305-403A maps to a region between residue 147 to 197 of human Prefoldin subunit 3 using the numbering given in entry P61758.3 (GeneID 7411).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Von Hippel-Lindau-binding protein 1 (VBP1) binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. VPB1 binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P61758].
Alternate Names
HIBBJ46; PFD3; PFDN3; prefoldin subunit 3; VBP-1; VHL-binding protein 1; von Hippel-Lindau binding protein 1; von Hippel-Lindau-binding protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.