Bethyl Laboratories
Rabbit anti-VRK1 Antibody Affinity Purified
Primary
Catalog #A305-268A
TargetVRK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Vaccinia-related kinase 1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases.VRK1 is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This protein, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN [taken from NCBI Entrez Gene (Gene ID: 7443)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetVRK1
Antigen Species
Human
Gene ID7443
Gene Symbol
VRK1
Gene Name
vaccinia related kinase 1
Uniprot IDQ99986
Protein Name
Serine/threonine-protein kinase VRK1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to VRK1 immobilized on solid support.
The epitope recognized by A305-268A maps to a region between residue 1 to 50 of human Serine/threonine-protein kinase VRK1 using the numbering given in entry Q99986.1 (GeneID 7443).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-268A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-268A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-268AOpen
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