Bethyl Laboratories
Rabbit anti-WDR42A Antibody Affinity Purified
Primary
Catalog #A301-557A
TargetWDR42A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
WDR42A (WD repeat-containing protein 42A) contains seven WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. The function of WDR42A has not been characterized.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 547 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWDR42A
Antigen Species
Human
Gene ID50717
Gene Symbol
DCAF8
Gene Name
DDB1 and CUL4 associated factor 8
Uniprot IDQ5TAQ9
Protein Name
DDB1- and CUL4-associated factor 8
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR42A immobilized on solid support.
The epitope recognized by A301-557A maps to a region between residue 500 and 550 of human WD repeat domain 42A using the numbering given in entry NP_056541.2 (GeneID 50717).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-557A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-557A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-557AOpen
Citations / Publications
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