Rabbit anti-WIPI2 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A305-324A maps to a region between residue 404 to 454 of human WD repeat domain phosphoinositide-interacting protein 2 using the numbering given in entry Q9Y4P8.1 (GeneID 26100).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to WIPI2 immobilized on solid support.
The epitope recognized by A305-324A-T maps to a region between residue 404 to 454 of human WD repeat domain phosphoinositide-interacting protein 2 using the numbering given in entry Q9Y4P8.1 (GeneID 26100).
Additional Product Information
WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) is an early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P).WIPI2 may bind PtdIns3P. [taken from www.uniprot.org/uniprot/Q9Y4P8].
Alternate Names
ATG18B; Atg21; CGI-50; WD repeat domain phosphoinositide-interacting protein 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI49-like protein 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.