Bethyl Laboratories
Rabbit anti-WNK3 Antibody Affinity Purified
Primary
Catalog #A301-877A
TargetWNK3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
WNK3 (With No Lysine 3) is a member of the WNK family of serine-threonine kinases which includes WNK1, WNK2, WNK3, and WNK4. The WNK family of kinases has been observed to play a role in several cellular processes which include ion balance, signal transduction, survival, proliferation and organ development. WNK3 appears to play a role in NaCl, water, and blood pressure homeostasis but has properties distinct from the other WNK family members. WNK3 is expressed throughout the nephron, particularly at intercellular junctions, and its kinase activity has been shown to regulate the activity of ion transporters such as the Na-K-2Cl and Na-Cl co-transporters.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetWNK3
Antigen Species
Human
Gene ID65267
Gene Symbol
WNK3
Gene Name
WNK lysine deficient protein kinase 3
Uniprot IDQ9BYP7
Protein Name
Serine/threonine-protein kinase WNK3
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WNK3 immobilized on solid support.
The epitope recognized by A301-877A maps to a region between residue 1 and 50 of human WNK lysine deficient protein kinase 3 using the numbering given in entry NP_001002838.1 (GeneID 65267).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-877A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-877A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-877AOpen
Citations / Publications
Services
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