Rabbit anti-WNK3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-877A maps to a region between residue 1 and 50 of human WNK lysine deficient protein kinase 3 using the numbering given in entry NP_001002838.1 (GeneID 65267).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to WNK3 immobilized on solid support.
The epitope recognized by A301-877A-T maps to a region between residue 1 and 50 of human WNK lysine deficient protein kinase 3 using the numbering given in entry NP_001002838.1 (GeneID 65267).
Additional Product Information
WNK3 (With No Lysine 3) is a member of the WNK family of serine-threonine kinases which includes WNK1, WNK2, WNK3, and WNK4. The WNK family of kinases has been observed to play a role in several cellular processes which include ion balance, signal transduction, survival, proliferation and organ development. WNK3 appears to play a role in NaCl, water, and blood pressure homeostasis but has properties distinct from the other WNK family members. WNK3 is expressed throughout the nephron, particularly at intercellular junctions, and its kinase activity has been shown to regulate the activity of ion transporters such as the Na-K-2Cl and Na-Cl co-transporters.
Alternate Names
PRKWNK3; Protein kinase lysine-deficient 3; protein kinase with no lysine 3; serine/threonine-protein kinase WNK3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.