Rabbit anti-Ymer Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-094A maps to a region between residue 125 and 175 of human Ymer (coiled-coil domain containing 50) using the numbering given in entry NP_777568.1 (GeneID 152137).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Ymer immobilized on solid support.
The epitope recognized by A302-094A-T maps to a region between residue 125 and 175 of human Ymer (coiled-coil domain containing 50) using the numbering given in entry NP_777568.1 (GeneID 152137).
Additional Product Information
Ymer was identified in a mass spectrometric study of epidermal growth factor signaling. It is a cytoplasmic tyrosine-phosphorylated protein that bears multiple ubiquitin-interacting domains. Recently Ymer has been implicated as a negative regulator of the NF-kappaB signaling pathway. Mutations in the gene that encodes Ymer, CCDC50, are the cause of autosomal dominant non-syndromic sensorineural deafness type 44.
Alternate Names
C3orf6; coiled-coil domain-containing protein 50; DFNA44; protein Ymer; YMER
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.