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Rabbit anti-ZCCHC8 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

ZCCHC8

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ZCCHC8 immobilized on solid support.

The epitope recognized by A301-805A-T maps to a region between residue 1 and 50 of human zinc finger, CCHC domain containing 8 using the numbering given in entry NP_060082.2 (GeneID 55596).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ZCCHC8 immobilized on solid support.

The epitope recognized by A301-805A maps to a region between residue 1 and 50 of human zinc finger, CCHC domain containing 8 using the numbering given in entry NP_060082.2 (GeneID 55596).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

ZCCHC8 is a zinc-finger protein that bears a zinc-binding motif composed of a CX2CX4HX4C sequence (where X can be any amino acid). ZCCHC8 also bears a PSP domain, a proline rich domain found in numerous splicesome associated proteins. ZCCHC8 has been identified as a component of the splicesome C complex and as a substrate of glycogen synthase kinase-3. It is suggested to play a role in the processing and metabolism of RNA.

55596

ZCCHC8

zinc finger CCHC-type containing 8

Q6NZY4

Zinc finger CCHC domain-containing protein 8

Alternate Names

TRAMP-like complex RNA-binding factor ZCCHC8; zinc finger CCHC domain-containing protein 8; zinc finger, CCHC domain containing 8

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.