Rabbit anti-ZEB2/SIP IHC Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by IHC-00691 maps to a region between residue 750 and 800 of human Smad interacting-protein 1 using the numbering given in entry NP_055610.1 (GeneID 9839).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ZEB2/SIP immobilized on solid support.
The epitope recognized by IHC-00691-T maps to a region between residue 750 and 800 of human Smad interacting-protein 1 using the numbering given in entry NP_055610.1 (GeneID 9839).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60315].
Alternate Names
HSPC082; SIP1; SIP-1; SMAD interacting protein 1; Smad-interacting protein 1; SMADIP1; ZFHX1B; zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.