Bethyl Laboratories
Rabbit anti-ZNF638/NP220 Antibody Affinity Purified
Primary
Catalog #A301-548A
TargetZNF638/NP220
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
ZNF638/NP220 is a Cys2-His2 zinc-finger DNA-binding protein that also bears repeats of an RNA recognition motif (RRM). ZNF638/NP220 has been reported to be structurally and functionally similar to matrin 3, a nuclear matrix protein involved in RNA processing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1928 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetZNF638/NP220
Antigen Species
Human
Gene ID27332
Gene Symbol
ZNF638
Gene Name
zinc finger protein 638
Uniprot IDQ14966
Protein Name
Zinc finger protein 638
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZNF638/NP220 immobilized on solid support.
The epitope recognized by A301-548A maps to a region between residue 1928 and 1978 of human zinc finger protein 638 (Nuclear protein 220) using the numbering given in entry NP_055312.2 (GeneID 27332).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-548A · Lot 4 (current lot)Open
Product DatasheetPDF · A301-548A · Lot 4 (current lot)Open
Safety Data SheetPDF · A301-548AOpen
Citations / Publications
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