Rabbit anti-ZNF638/NP220 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-548A maps to a region between residue 1928 and 1978 of human zinc finger protein 638 (Nuclear protein 220) using the numbering given in entry NP_055312.2 (GeneID 27332).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ZNF638/NP220 immobilized on solid support.
The epitope recognized by A301-548A-T maps to a region between residue 1928 and 1978 of human zinc finger protein 638 (Nuclear protein 220) using the numbering given in entry NP_055312.2 (GeneID 27332).
Additional Product Information
ZNF638/NP220 is a Cys2-His2 zinc-finger DNA-binding protein that also bears repeats of an RNA recognition motif (RRM). ZNF638/NP220 has been reported to be structurally and functionally similar to matrin 3, a nuclear matrix protein involved in RNA processing.
Alternate Names
CTCL tumor antigen se33-1; CTCL-associated antigen se33-1; cutaneous T-cell lymphoma-associated antigen se33-1; NP220; NP220 nuclear protein; nuclear protein 220; ZFML; Zfp638; zinc finger matrin-like protein; zinc finger protein 638
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.