F(ab')2 Goat anti-Mouse IgG-F(ab')2 Fragment Cross-Adsorbed Antibody Affinity Purified

Specifications

Production & Epitope

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to Mouse IgG-F(ab')2 Fragment was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.


By immunoelectrophoresis and ELISA this antibody reacts specifically with Fab fragment of mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with Fab fragments from other species.

Applications

Not all listed applications have been specifically tested by our laboratory.