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F(ab')2 Goat anti-Rabbit IgG-F(ab')2 Fragment Cross-Adsorbed Antibody Biotinylated
Bethyl Laboratories
Catalog #
Target:
Rabbit IgG-F(ab')2 Fragment
Reactivity:
Rabbit
Applications:
ELISA,
Flow Cyt,
ICC,
IF,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
F(ab')2
Isotype:
IgG
Conjugate:
Biotin,
DyLight® 488,
DyLight® 650,
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
DyLight® 488 (0.5 mg)
DyLight® 650 (0.5 mg)
HRP (0.5 mg)
$140.00
$197.00
Product Details
Specifications
Verified Reactivity
Rabbit
Antigen Species
Rabbit
Minimal Reactivity
Human, Mouse, Rat
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG-F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. F(ab')2 fragments were conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of rabbit IgG. No antibody was detected against non-immunoglobulin serum proteins. Cross reactivity with IgA and IgM is negligible. Less than 1% cross reactivity to human, mouse and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species.
Country of Origin
USA
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-F(ab')2 activity ensures specificity for the intact heavy- and light-chain complex independent from the Fc region.
Applications
Not all listed applications have been specifically tested by our laboratory.
DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.