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F(ab')2 Rabbit anti-Mouse IgG-Heavy and Light Chain Cross-Adsorbed Antibody Biotinylated

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A90-243B

Target:
Mouse IgG-heavy and light chain
Reactivity:
Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
F(ab')2
IsoType:
IgG
Conjugate:
Biotin
Purity:
Antigen Affinity Purified
0.5 mg

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Minimum Reactivity Species
human,rat
Stock Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prod Procedures
Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to biotin.

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

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Applications

Not all listed applications have been specifically tested by our laboratory.