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Goat anti-Human IgG-Fab Fragment Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Target:
Human IgG-Fab Fragment
Reactivity:
Human
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified, Antiserum
Antiserum (2 ml)

Unconjugated (1 mg)

$73.00 $100.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Serum containing 0.09% Sodium Azide
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Production Procedures
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with Fab fragment of human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with Fab fragments from other species.
Antiserum was solid phase adsorbed to ensure specificity.

By immunoelectrophoresis, the antibody reacts specifically with Fab fragment of human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

This antibody may cross react with Fab fragments from other species.
Country of Origin
USA

Additional Product Information

IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fab activity ensures specificity for a single arm of the Fab fragment and not the Fc portion of the antibody.

Applications

Not all listed applications have been specifically tested by our laboratory. For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).

Optimal working dilutions should be determined experimentally by the investigator.