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Goat anti-Human IgG+IgM Heavy and Light Chain Antibody Biotinylated
Bethyl Laboratories
Catalog #
Target:
Human IgG+IgM-Heavy and Light Chain
Reactivity:
Human
Applications:
ELISA,
Flow Cyt,
ICC,
IF,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin,
FITC,
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Verified Reactivity
Human
Antigen Species
Human
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
The antibody to pig IgG was isolated by affinity chromatography using antigens coupled to agarose beads and conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Country of Origin
USA
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. IgM is typically referred to as a pentamer, containing 5 monomers held together by a J chain protein. IgM is found primarily in serum and is the first part of the antibody response to infection.
Applications
Not all listed applications have been specifically tested by our laboratory.