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Goat anti-Human IgG+IgM+IgA Heavy and Light Chain Antibody Biotinylated
Bethyl Laboratories
Catalog #
Target:
Human IgG+IgM+IgA Heavy and Light Chain
Reactivity:
Human
Applications:
ELISA
,Flow Cyt
,ICC
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (1 mg)
Biotin (1 mg)
FITC (1 mg)
HRP (1 mg)
Product Details
Specifications
Verified Reactivity
Human
Antigen Species
Human
Concentration
1 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
The antibody was isolated by affinity chromatography using antigens coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG, IgA and IgM and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma and functions to neutralize toxins and cell-mediated cytotoxicity. IgM is typically referred to as a pentamer, and is found primarily in serum and is the first part of the antibody response to infection. IgA is an immunoglobulin that is typically dimeric and held together by a J-chain protein. IgA is typically found near the mucous membranes of the body, including the gut, respiratory system, eyes, and colostrum.
Applications
