NEW!
Goat anti-Human Kappa Light Chain Antibody Biotinylated
Bethyl Laboratories
Catalog #
Target:
Human Kappa Light Chain
Reactivity:
Human
Applications:
DD,
ELISA,
ICC,
IEP,
IF,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin,
FITC,
HRP,
Unconjugated
Purity:
Antigen Affinity Purified,
IgG Fraction
IgG Fraction (2 ml)
Unconjugated (1 mg)
Biotin (1 mg)
FITC (1 mg)
HRP (1 mg)
$90.00
$163.00
Product Details
Specifications
Verified Reactivity
Human
Antigen Species
Human
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda light chain or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. Antiserum was solid phase adsorbed to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
Total protein was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody titer determined by reverse RID: 2 mg/ml
By immunoelectrophoresis this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda light chain or non-immunoglobulin serum proteins.
This antibody may cross react with kappa light chain from other species.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda light chain or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda or non-immunoglobulin serum proteins. This antibody may cross react with kappa light chain from other species. Antiserum was solid phase adsorbed to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
Total protein was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody titer determined by reverse RID: 2 mg/ml
By immunoelectrophoresis this antibody reacts specifically with kappa light chains common to all human immunoglobulins. No antibody was detected against lambda light chain or non-immunoglobulin serum proteins.
This antibody may cross react with kappa light chain from other species.
Country of Origin
USA
Additional Product Information
The kappa light chain is a component of an Ig molecule that typically forms an Ig molecule linking to a heavy chain. The human body makes both kappa and lambda light chains, and elevated kappa levels may suggest disorders such as amyloidosis or multiple myeloma.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP), double diffusion Ouchterlony (DD) or immunofixation electrophoresis (IFE)).
Optimal working dilutions should be determined experimentally by the investigator.