Goat anti-Mouse Lambda Light Chain Antibody HRP Conjugated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with lambda light chain common to all mouse immunoglobulins. No antibody was detected against kappa or non-immunoglobulin serum proteins. This antibody may cross react with lambda from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with lambda light chain common to all mouse immunoglobulins. No antibody was detected against kappa or non-immunoglobulin serum proteins.
This antibody may cross react with lambda from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with lambda light chain common to all mouse immunoglobulins. No antibody was detected against kappa or non-immunoglobulin serum proteins.
This antibody may cross react with lambda from other species.
Additional Product Information
The lambda light chain is a component of an Ig molecule that typically forms an Ig molecule linking to a heavy chain. The human body makes both kappa and lambda light chains, and elevated lambda levels may suggest disorders such as amyloidosis or multiple myeloma.
Applications
Not all listed applications have been specifically tested by our laboratory.