Bethyl Laboratories
Goat anti-Rabbit IgG-Fc Fragment Antibody Affinity Purified
Secondary
Catalog #A120-111A
TargetRabbit IgG-Fc Fragment
HostGoat
ClonalityPolyclonal
ReactivityRabbit
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Rabbit
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis, this antibody reacts specifically with rabbit IgG. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A120-111AOpen
Product DatasheetPDF · A120-111A · Lot 240606 (current lot)Open
Safety Data SheetPDF · A120-111AOpen
Product DatasheetPDF · A120-111A · Lot 17Open
Safety Data SheetPDF · A120-111AOpen
Product DatasheetPDF · A120-111A · Lot 16Open
Safety Data SheetPDF · A120-111AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
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