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Goat anti-Rat IgG-Fc Fragment Cross-Adsorbed Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Rat IgG-Fc Fragment
Reactivity:
Rat
Applications:
ELISA,
Flow Cyt,
ICC,
IF,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin,
DyLight® 488,
DyLight® 550,
DyLight® 594,
DyLight® 650,
FITC,
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
DyLight® 488 (0.5 mg)
DyLight® 550 (0.5 mg)
DyLight® 594 (0.5 mg)
DyLight® 650 (0.5 mg)
FITC (0.5 mg)
HRP (0.5 mg)
$106.00
$174.00
Product Details
Specifications
Verified Reactivity
Rat
Antigen Species
Rat
Minimal Reactivity
Human, Mouse
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Biotin/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
Biotinylated antibody was demonstrated by reaction with avidin/peroxidase when coated on microtiter wells. Working dilutions should be determined by the investigator. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme: antibody protein ratio is 1:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Biotin/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
Biotinylated antibody was demonstrated by reaction with avidin/peroxidase when coated on microtiter wells. Working dilutions should be determined by the investigator. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme: antibody protein ratio is 1:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
Country of Origin
USA
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Applications
Not all listed applications have been specifically tested by our laboratory.
DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.