Goat anti-Rat IgG-Fc Fragment Cross-Adsorbed Antibody Affinity Purified

Specifications

Production & Epitope

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Less than 2% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.

Applications

Not all listed applications have been specifically tested by our laboratory.