Rabbit anti-Human IgG-Fc Fragment Antibody Affinity Purified
Product Details
Specifications
Request Formulation Change Serum containing 0.09% Sodium Azide
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Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA, IgM and light chains is less than 1%.
This antibody may cross react with IgG from other species. Antiserum was solid phase adsorbed to ensure class specificity.
By immunoelectrophoresis, the antiserum reacts specifically with Human IgG-Fc Fragment. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.